Diane S. Lidke, PhD

Assistant Professor, Department of Pathology

 

Contact information:

Diane S. Lidke. Ph.D.
Dept. of Pathology
Cancer Research Facility, Room 203
University of New Mexico School of Medicine
Albuquerque, New Mexico 87131
dlidke@salud.unm.edu
(505) 272-8375 (office)
(505) 272-1435 (fax)

        
         

 

Dynamics of EGF (Epidermal Growth Factor) binding to the membrane receptor, EGFR.

A431 cell expressing EGFR-GFP (green) after addition of EGF labeled Quantum Dots (EGF-QDs, red). The EGF-QDs bind to the surface and are internalize through receptor mediated endocystosis.

 
Retrograde transport of EGF-QDs on filopodia. An A431 cell expressing erbB3-Citrine (green). EGF-QDs (red) bind to the cell, including the filopodia from which they are transported to the cell body. (Lidke et al. Nat Biotech22: 198)

 
  Tracking a single EGF-QD-EGFR complex (red) during retrograde transport along a filipodium. Left: selected frames from a time series. Right: trajectory of single complex marked by yellow arrow. (Lidke et al. JCB170:619)      
 

 

Research Interests

The responses of a cell to its surrounding environment result from uptake of signaling mediators through the membrane and the transduction of signals from the cell surface to the nucleus. Strict regulation of signal transduction is crucial for proper cellular function, such as gene expression, cell migration and cell death. Many diseases and cancers are attributed to unregulated signaling pathways. However, many aspects of how the cell maintains spatio-temporal control of signal transduction still remain unclear.

Correlating protein activity with structure, aggregation state and localization is essential for understanding cell function. Examining proteins in live cells using high-resolution bio-imaging methods and biophysical techniques can reveal information about the dynamics of protein interactions. Fluorescence imaging microscopy not only complements the existing biochemical, genetic and physiological techniques for studying cellular function, but also adds information on localization and temporal resolution. Methods such as real-time visualization, FRET (Fluorescence Resonance Energy Transfer) microscopy, FRAP (Fluorescence Recovery After Photobleaching), single molecule tracking and anisotropy imaging can help to shed light on the underlying questions of cell signaling mechanisms.

My research interests involve development and application of new techniques in fluorescence microscopy that combine the spatial, temporal and spectroscopic properties of a sample for use in live cell imaging, with an emphasis on the study of protein dynamics and interactions during signal transduction.

 

Selected Publications

D.S. Lidke, P. Nagy, R. Heintzmann, D.J. Arndt-Jovin, J.N. Post, H. Grecco, E.A. Jares-Erijman and T.M. Jovin."Quantum Dot ligands provide new insights into receptor-mediated signal transduction." 2004. Nature Biotechnology, 22: 198-203.

D.S. Lidke, K.A. Lidke, B. Rieger, T.M. Jovin and D.J. Arndt-Jovin. "Reaching out for signals: filopodia sense EGF and respond by directed retrograde transport of activated receptors." 2005. Journal of Cell Biology, 170: 619-626.

K.A. Lidke, B. Reiger, D.S. Lidke and T.M. Jovin. "The role of photon statistics in fluorescence anisotropy imaging." 2005. IEEE Journal for Image Processing. 14: 1237-1245.

D.S. Lidke, P. Nagy, B.G. Barisas, R. Heintzmann, J.N. Post, K.A. Lidke, A.H.A. Clayton, D.J. Arndt-Jovin and T.M. Jovin . "Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)." 2003. Biochemical Society Transactions,31: 1020-1027.

D.S. Lidke and D.D. Thomas. "Coordination of the two heads of myosin during muscle contraction." 2002. Proceedings of the National Academy of Sciences of the United States of America ,99: 14801-14806.

D.S. Lidke and D.J. Arndt-Jovin. "Imaging takes a quantum leap." 2004. An invited review for Physiology, 19: 322-325.